The Federal Regulations Code specifies rules for collecting drug test specimens from DOT employees. Employees selected for random drug testing must submit urine specimens at a specified collection site. Tests are conducted by a certified collector. The test administrator must begin the collection process soon after the employee enters the collection site.

Before submitting a urine specimen, the employee must present employer-issued photo identification, remove all outer clothing and empty his pockets so that the collector can verify that the employee is not concealing any items or substances that could be used to tamper with the specimen.